2V3J

The yeast ribosome synthesis factor Emg1 alpha beta knot fold methyltransferase

2V3J の概要

• エントリーDOI: 10.2210/pdb2v3j/pdb
• 関連するPDBエントリー: 2V3K
• 分子名称: ESSENTIAL FOR MITOTIC GROWTH 1, SULFATE ION (3 entities in total)
• 機能のキーワード: emg1, rrna processing, nuclear protein, ribonucleoprotein, ribosome biogenesis, alpha/beta knot fold methyltransferase, ribosomal protein
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29238.83

構造登録者

Leulliot, N.,Bohnsack, M.T.,Graille, M.,Tollervey, D.,Van Tilbeurgh, H. (登録日: 2007-06-18, 公開日: 2007-12-04, 最終更新日: 2024-10-16)

主引用文献

Leulliot, N.,Bohnsack, M.T.,Graille, M.,Tollervey, D.,Van Tilbeurgh, H.
The Yeast Ribosome Synthesis Factor Emg1 is a Novel Member of the Superfamily of Alpha/Beta Knot Fold Methyltransferases.
Nucleic Acids Res., 36:629-, 2008

PubMed Abstract: Emg1 was previously shown to be required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. Here we report the determination of the crystal structure of Emg1 at 2 A resolution in complex with the methyl donor, S-adenosyl-methionine (SAM). This structure identifies Emg1 as a novel member of the alpha/beta knot fold methyltransferase (SPOUT) superfamily. In addition to the conserved SPOUT core, Emg1 has two unique domains that form an extended surface, which we predict to be involved in binding of RNA substrates. A point mutation within a basic patch on this surface almost completely abolished RNA binding in vitro. Three point mutations designed to disrupt the interaction of Emg1 with SAM each caused>100-fold reduction in SAM binding in vitro. Expression of only Emg1 with these mutations could support growth and apparently normal ribosome biogenesis in strains genetically depleted of Emg1. We conclude that the catalytic activity of Emg1 is not essential and that the presence of the protein is both necessary and sufficient for ribosome biogenesis.

PubMed: 18063569
DOI: 10.1093/NAR/GKM1074

実験手法

X-RAY DIFFRACTION (2 Å)