2V1X

Crystal structure of human RECQ-like DNA helicase

2V1X の概要

• エントリーDOI: 10.2210/pdb2v1x/pdb
• 分子名称: ATP-DEPENDENT DNA HELICASE Q1, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: dna strand annealing, mismatch repair, nucleotide-binding, dna-binding, nuclear protein, atpase, helicase, hydrolase, atp-binding
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus : P46063
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136246.92

構造登録者

Pike, A.C.W.,Shrestha, B.,Burgess-Brown, N.,King, O.,Ugochukwu, E.,Watt, S.,Edwards, A.,Arrowsmith, C.H.,Weigelt, J.,Sundstrom, M.,Gileadi, O. (登録日: 2007-05-30, 公開日: 2007-07-03, 最終更新日: 2023-12-13)

主引用文献

Pike, A.C.W.,Shrestha, B.,Popuri, V.,Burgess-Brown, N.,Muzzolini, L.,Costantini, S.,Vindigni, A.,Gileadi, O.
Structure of the Human Recq1 Helicase Reveals a Putative Strand-Separation Pin.
Proc.Natl.Acad.Sci.USA, 106:1039-, 2009

PubMed Abstract: RecQ-like helicases, which include 5 members in the human genome, are important in maintaining genome integrity. We present a crystal structure of a truncated form of the human RECQ1 protein with Mg-ADP. The truncated protein is active in DNA fork unwinding but lacks other activities of the full-length enzyme: disruption of Holliday junctions and DNA strand annealing. The structure of human RECQ1 resembles that of Escherichia coli RecQ, with some important differences. All structural domains are conserved, including the 2 RecA-like domains and the RecQ-specific zinc-binding and winged-helix (WH) domains. However, the WH domain is positioned at a different orientation from that of the E. coli enzyme. We identify a prominent beta-hairpin of the WH domain as essential for DNA strand separation, which may be analogous to DNA strand-separation features of other DNA helicases. This hairpin is significantly shorter in the E. coli enzyme and is not required for its helicase activity, suggesting that there are significant differences between the modes of action of RecQ family members.

PubMed: 19151156
DOI: 10.1073/PNAS.0806908106

実験手法

X-RAY DIFFRACTION (2 Å)