2V08

Structure of wild-type Phormidium laminosum cytochrome c6

2V08 の概要

• エントリーDOI: 10.2210/pdb2v08/pdb
• 分子名称: CYTOCHROME C6, HEME C, IMIDAZOLE, ... (6 entities in total)
• 機能のキーワード: cytochrome, photosynthesis, cyano-bacteria, electron-transfer
• 由来する生物種: PHORMIDIUM LAMINOSUM
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20052.71

構造登録者

Worrall, J.A.R.,Schlarb-Ridley, B.G.,Reda, T.,Marcaida, M.J.,Moorlen, R.J.,Wastl, J.,Hirst, J.,Bendall, D.S.,Luisi, B.F.,Howe, C.J. (登録日: 2007-05-10, 公開日: 2007-07-24, 最終更新日: 2024-11-13)

主引用文献

Worrall, J.A.,Schlarb-Ridley, B.G.,Reda, T.,Marcaida, M.J.,Moorlen, R.J.,Wastl, J.,Hirst, J.,Bendall, D.S.,Luisi, B.F.,Howe, C.J.
Modulation of heme redox potential in the cytochrome c6 family.
J. Am. Chem. Soc., 129:9468-9475, 2007

PubMed Abstract: Cytochrome c6A is a unique dithio-cytochrome of green algae and plants. It has a very similar core structure to that of bacterial and algal cytochromes c6 but is unable to fulfill the same function of transferring electrons from cytochrome f to photosystem I. A key feature is that its heme midpoint potential is more than 200 mV below that of cytochrome c6 despite having His and Met as axial heme-iron ligands. To identify the molecular origins of the difference in potential, the structure of cytochrome c6 from the cyanobacterium Phormidium laminosum has been determined by X-ray crystallography and compared with the known structure of cytochrome c6A. One salient difference of the heme pockets is that a highly conserved Gln (Q51) in cytochrome c6 is replaced by Val (V52) in c6A. Using protein film voltammetry, we found that swapping these residues raised the c6A potential by +109 mV and decreased that of c6 by almost the same extent, -100 mV. X-ray crystallography of the V52Q protein showed that the Gln residue adopts the same configuration relative to the heme as in cytochrome c6 and we propose that this stereochemistry destabilizes the oxidized form of the heme. Consequently, replacement of Gln by Val was probably a key step in the evolution of cytochrome c6A from cytochrome c6, inhibiting reduction by the cytochrome b6f complex and facilitating establishment of a new function.

PubMed: 17625855
DOI: 10.1021/ja072346g

実験手法

X-RAY DIFFRACTION (2 Å)