2SEB

X-RAY CRYSTAL STRUCTURE OF HLA-DR4 COMPLEXED WITH A PEPTIDE FROM HUMAN COLLAGEN II

Summary for 2SEB

• Entry DOI: 10.2210/pdb2seb/pdb
• Descriptor: HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, ENTEROTOXIN TYPE B, PEPTIDE FROM COLLAGEN II, ... (6 entities in total)
• Functional Keywords: complex (mhc class ii-superantigen), complex (mhc class ii-superantigen) complex, complex (mhc class ii/superantigen)
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 73362.36

Authors

Dessen, A.,Lawrence, C.M.,Cupo, S.,Zaller, D.M.,Wiley, D.C. (deposition date: 1997-10-16, release date: 1998-01-28, Last modification date: 2024-10-23)

Primary citation

Dessen, A.,Lawrence, C.M.,Cupo, S.,Zaller, D.M.,Wiley, D.C.
X-ray crystal structure of HLA-DR4 (DRA*0101, DRB1*0401) complexed with a peptide from human collagen II.
Immunity, 7:473-481, 1997

PubMed Abstract: Genetic predisposition to rheumatoid arthritis (RA) is linked to the MHC class II allele HLA-DR4. The charge of the amino acid at DRbeta71 in the peptide-binding site appears to be critical in discriminating DR molecules linked to increased disease susceptibility. We have determined the 2.5 A x-ray structure of the DR4 molecule with the strongest linkage to RA (DRB1*0401) complexed with a human collagen II peptide. Details of a predicted salt bridge between lysine DRbeta71 and aspartic acid at the P4 peptide position suggest how it may participate in both antigen binding and TCR activation. A model is proposed for the DR4 recognition of collagen II (261-273), an antigen immunodominant in human-transgenic mouse models of RA.

PubMed: 9354468
DOI: 10.1016/S1074-7613(00)80369-6

Experimental method

X-RAY DIFFRACTION (2.5 Å)