2RR3

Solution structure of the complex between human VAP-A MSP domain and human OSBP FFAT motif

Summary for 2RR3

• Entry DOI: 10.2210/pdb2rr3/pdb
• Descriptor: Vesicle-associated membrane protein-associated protein A, Oxysterol-binding protein 1 (2 entities in total)
• Functional Keywords: lipid transport, transport, protein-peptide complex, major sperm protein domain, protein binding, endoplasmic reticulum, lipid binding, membrane protein-transport protein complex, membrane protein/transport protein
• Biological source: Homo sapiens (human); More
• Cellular location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein: Q9P0L0; Cytoplasm: P22059
• Total number of polymer chains: 2
• Total formula weight: 20184.73

Authors

Furuita, K.,Jee, J.,Fukada, H.,Mishima, M.,Kojima, C. (deposition date: 2010-03-09, release date: 2010-03-23, Last modification date: 2024-05-01)

Primary citation

Furuita, K.,Jee, J.,Fukada, H.,Mishima, M.,Kojima, C.
Electrostatic interaction between oxysterol-binding protein and VAMP-associated protein A revealed by NMR and mutagenesis studies
J.Biol.Chem., 285:12961-12970, 2010

PubMed Abstract: Oxysterol-binding protein (OSBP), a cytosolic receptor of cholesterol and oxysterols, is recruited to the endoplasmic reticulum by binding to the cytoplasmic major sperm protein (MSP) domain of integral endoplasmic reticulum protein VAMP-associated protein-A (VAP-A), a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol. To delineate the interaction mechanism between VAP-A and OSBP, we determined the complex structure between the VAP-A MSP domain (VAP-A(MSP)) and the OSBP fragment containing a VAP-A binding motif FFAT (OSBP(F)) by NMR. This solution structure explained that five of six conserved residues in the FFAT motif are required for the stable complex formation, and three of five, including three critical intermolecular electrostatic interactions, were not explained before. By combining NMR relaxation and titration, isothermal titration calorimetry, and mutagenesis experiments with structural information, we further elucidated the detailed roles of the FFAT motif and underlying motions of VAP-A(MSP), OSBP(F), and the complex. Our results show that OSBP(F) is disordered in the free state, and VAP-A(MSP) and OSBP(F) form a final complex by means of intermediates, where electrostatic interactions through acidic residues, including an acid patch preceding the FFAT motif, probably play a collective role. Additionally, we report that the mutation that causes the familial motor neuron disease decreases the stability of the MSP domain.

PubMed: 20178991
DOI: 10.1074/jbc.M109.082602

Experimental method

SOLUTION NMR