Crystal Structure of S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400

Replaces:  2PU6

Summary for 2RI6

Related2OG1 1J1I 1U2E 2PU7 1C4X 2D0D
Descriptor2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, SODIUM ION, MALONATE ION, ... (4 entities in total)
Functional Keywordshydrolase, c-c bond hydrolase, aromatic hydrocarbons catabolism
Biological sourceBurkholderia xenovorans
Total number of polymer chains1
Total molecular weight31976.35
Bhowmik, S.,Bolin, J.T. (deposition date: 2007-10-10, release date: 2007-11-06, Last modification date: 2017-10-25)
Primary citation
Horsman, G.P.,Bhowmik, S.,Seah, S.Y.,Kumar, P.,Bolin, J.T.,Eltis, L.D.
The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad.
J.Biol.Chem., 282:19894-19904, 2007
PubMed: 17442675 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M702237200
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.20430.4%2.6%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report