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2RHS

PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies

2RHS の概要
エントリーDOI10.2210/pdb2rhs/pdb
関連するPDBエントリー2RHQ
分子名称Phenylalanyl-tRNA synthetase alpha chain, Phenylalanyl-tRNA synthetase beta chain, 1-{3-[(4-pyridin-2-ylpiperazin-1-yl)sulfonyl]phenyl}-3-(1,3-thiazol-2-yl)urea, ... (6 entities in total)
機能のキーワードheterotetramer, phenylalanine, trna, aminoacyl-trna synthetase, atp-binding, cytoplasm, ligase, magnesium, metal-binding, nucleotide-binding, protein biosynthesis, rna-binding, trna-binding
由来する生物種Staphylococcus haemolyticus
詳細
細胞内の位置Cytoplasm : Q4L5E3 Q4L5E4
タンパク質・核酸の鎖数4
化学式量合計245446.04
構造登録者
Evdokimov, A.G.,Mekel, M. (登録日: 2007-10-09, 公開日: 2007-11-06, 最終更新日: 2024-02-21)
主引用文献Evdokimov, A.G.,Mekel, M.,Hutchings, K.,Narasimhan, L.,Holler, T.,McGrath, T.,Beattie, B.,Fauman, E.,Yan, C.,Heaslet, H.,Walter, R.,Finzel, B.,Ohren, J.,McConnell, P.,Braden, T.,Sun, F.,Spessard, C.,Banotai, C.,Al-Kassim, L.,Ma, W.,Wengender, P.,Kole, D.,Garceau, N.,Toogood, P.,Liu, J.
Rational protein engineering in action: the first crystal structure of a phenylalanine tRNA synthetase from Staphylococcus haemolyticus.
J.Struct.Biol., 162:152-169, 2008
Cited by
PubMed Abstract: In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog. We also explain the structure-activity relationship of several recently reported inhibitors. The native enzyme crystals were of poor quality--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.
PubMed: 18086534
DOI: 10.1016/j.jsb.2007.11.002
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.2 Å)
構造検証レポート
Validation report summary of 2rhs
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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