2RHS

PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies

2RHS の概要

• エントリーDOI: 10.2210/pdb2rhs/pdb
• 関連するPDBエントリー: 2RHQ
• 分子名称: Phenylalanyl-tRNA synthetase alpha chain, Phenylalanyl-tRNA synthetase beta chain, 1-{3-[(4-pyridin-2-ylpiperazin-1-yl)sulfonyl]phenyl}-3-(1,3-thiazol-2-yl)urea, ... (6 entities in total)
• 機能のキーワード: heterotetramer, phenylalanine, trna, aminoacyl-trna synthetase, atp-binding, cytoplasm, ligase, magnesium, metal-binding, nucleotide-binding, protein biosynthesis, rna-binding, trna-binding
• 由来する生物種: Staphylococcus haemolyticus; 詳細
• 細胞内の位置: Cytoplasm : Q4L5E3 Q4L5E4
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245446.04

構造登録者

Evdokimov, A.G.,Mekel, M. (登録日: 2007-10-09, 公開日: 2007-11-06, 最終更新日: 2024-02-21)

主引用文献

Evdokimov, A.G.,Mekel, M.,Hutchings, K.,Narasimhan, L.,Holler, T.,McGrath, T.,Beattie, B.,Fauman, E.,Yan, C.,Heaslet, H.,Walter, R.,Finzel, B.,Ohren, J.,McConnell, P.,Braden, T.,Sun, F.,Spessard, C.,Banotai, C.,Al-Kassim, L.,Ma, W.,Wengender, P.,Kole, D.,Garceau, N.,Toogood, P.,Liu, J.
Rational protein engineering in action: the first crystal structure of a phenylalanine tRNA synthetase from Staphylococcus haemolyticus.
J.Struct.Biol., 162:152-169, 2008

PubMed Abstract: In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog. We also explain the structure-activity relationship of several recently reported inhibitors. The native enzyme crystals were of poor quality--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.

PubMed: 18086534
DOI: 10.1016/j.jsb.2007.11.002

実験手法

X-RAY DIFFRACTION (2.2 Å)