2RFB

Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus

2RFB の概要

• エントリーDOI: 10.2210/pdb2rfb/pdb
• 関連するPDBエントリー: 2RFC
• 分子名称: Cytochrome P450, SULFATE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: cytochrome p450 thermophile, heme, iron, metal-binding, monooxygenase, oxidoreductase
• 由来する生物種: Picrophilus torridus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 121076.90

構造登録者

Ho, W.W.,Li, H.,Poulos, T.L.,Nishida, C.R.,Ortiz de Montellano, P.R. (登録日: 2007-09-28, 公開日: 2008-01-29, 最終更新日: 2024-02-21)

主引用文献

Ho, W.W.,Li, H.,Nishida, C.R.,Ortiz de Montellano, P.R.,Poulos, T.L.
Crystal Structure and Properties of CYP231A2 from the Thermoacidophilic Archaeon Picrophilus torridus.
Biochemistry, 47:2071-2079, 2008

PubMed Abstract: The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phenylimidazole. Comparison of the ligand-free and -bound CYP231A2 structures shows conformational changes where the F and G helices swing as a single rigid body about a pivot point at the N-terminal end of the F helix, allowing the F helix region to dip toward the heme, resulting in closer contacts with the ligand. Thermal melting data illustrate that the melting temperature for CYP231A2 increases nearly 10 degrees C upon ligand binding, thus illustrating that the closed conformation is substantially more stable. Furthermore, spectroscopic data indicate that the active site is stable at pH 4.5, although, unusually, the thiolate ligand to the iron can be reversibly protonated. CYP231A2 does not exhibit structural features normally associated with thermophilic proteins such as an increase in salt bridge networks or extensive aromatic clustering. The increase in thermal stability instead is best correlated with the smaller size and shorter loops in CYP231A2 compared to other P450s.

PubMed: 18197710
DOI: 10.1021/bi702240k

実験手法

X-RAY DIFFRACTION (2.5 Å)