2RE3

CRYSTAL STRUCTURE OF a DUF1285 family protein (SPO_0140) FROM SILICIBACTER POMEROYI DSS-3 AT 2.50 A RESOLUTION

Summary for 2RE3

• Entry DOI: 10.2210/pdb2re3/pdb
• Descriptor: Uncharacterized protein, GLYCEROL (3 entities in total)
• Functional Keywords: structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, unknown function
• Biological source: Silicibacter pomeroyi DSS-3
• Total number of polymer chains: 2
• Total formula weight: 43307.35

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2007-09-25, release date: 2007-10-09, Last modification date: 2024-10-09)

Primary citation

Han, G.W.,Bakolitsa, C.,Miller, M.D.,Kumar, A.,Carlton, D.,Najmanovich, R.J.,Abdubek, P.,Astakhova, T.,Axelrod, H.L.,Chen, C.,Chiu, H.J.,Clayton, T.,Das, D.,Deller, M.C.,Duan, L.,Ernst, D.,Feuerhelm, J.,Grant, J.C.,Grzechnik, A.,Jaroszewski, L.,Jin, K.K.,Johnson, H.A.,Klock, H.E.,Knuth, M.W.,Kozbial, P.,Krishna, S.S.,Marciano, D.,McMullan, D.,Morse, A.T.,Nigoghossian, E.,Okach, L.,Reyes, R.,Rife, C.L.,Sefcovic, N.,Tien, H.J.,Trame, C.B.,van den Bedem, H.,Weekes, D.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
Structures of the first representatives of Pfam family PF06938 (DUF1285) reveal a new fold with repeated structural motifs and possible involvement in signal transduction.
Acta Crystallogr.,Sect.F, 66:1218-1225, 2010

PubMed Abstract: The crystal structures of SPO0140 and Sbal_2486 were determined using the semiautomated high-throughput pipeline of the Joint Center for Structural Genomics (JCSG) as part of the NIGMS Protein Structure Initiative (PSI). The structures revealed a conserved core with domain duplication and a superficial similarity of the C-terminal domain to pleckstrin homology-like folds. The conservation of the domain interface indicates a potential binding site that is likely to involve a nucleotide-based ligand, with genome-context and gene-fusion analyses additionally supporting a role for this family in signal transduction, possibly during oxidative stress.

PubMed: 20944214
DOI: 10.1107/S1744309109050416

Experimental method

X-RAY DIFFRACTION (2.5 Å)