2R8P

Transketolase from E. coli in complex with substrate D-fructose-6-phosphate

Summary for 2R8P

• Entry DOI: 10.2210/pdb2r8p/pdb
• Related: 1QGD 2R5N 2R8O
• Descriptor: Transketolase 1, CALCIUM ION, 2-C-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium-2-yl}-6-O-phosphono-D-glucitol, ... (5 entities in total)
• Functional Keywords: reaction intermediate, calcium, metal-binding, thiamine pyrophosphate, transferase
• Biological source: Escherichia coli K12
• Total number of polymer chains: 2
• Total formula weight: 148252.68

Authors

Wille, G.,Asztalos, P.,Weiss, M.S.,Tittmann, K. (deposition date: 2007-09-11, release date: 2007-12-11, Last modification date: 2024-02-21)

• Primary citation: Asztalos, P.,Parthier, C.,Golbik, R.,Kleinschmidt, M.,Hubner, G.,Weiss, M.S.,Friedemann, R.,Wille, G.,Tittmann, K.; Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.; Biochemistry, 46:12037-12052, 2007; PubMed: 17914867; DOI: 10.1021/bi700844m

Experimental method

X-RAY DIFFRACTION (1.65 Å)