2QZT

Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti

Summary for 2QZT

• Entry DOI: 10.2210/pdb2qzt/pdb
• Related: 1PZ4
• Descriptor: Sterol carrier protein 2-like 2, PALMITIC ACID (3 entities in total)
• Functional Keywords: sterol carrier, mosquito, fatty acid, palmitic acid, cholesterol, lipid transport
• Biological source: Aedes aegypti (yellow fever mosquito)
• Total number of polymer chains: 2
• Total formula weight: 24130.32

Authors

Dyer, D.H.,Lan, Q.,Forest, K.T. (deposition date: 2007-08-17, release date: 2008-03-11, Last modification date: 2024-02-21)

Primary citation

Dyer, D.H.,Wessely, V.,Forest, K.T.,Lan, Q.
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members.
J.Lipid Res., 49:644-653, 2008

PubMed Abstract: Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [3H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.

PubMed: 18084051
DOI: 10.1194/jlr.M700460-JLR200

Experimental method

X-RAY DIFFRACTION (1.7 Å)