2QYN

Crystal structure of PDE4D2 in complex with inhibitor NPV

2QYN の概要

• エントリーDOI: 10.2210/pdb2qyn/pdb
• 関連するPDBエントリー: 2qyk 2qyl 2qym
• 分子名称: cAMP-specific 3',5'-cyclic phosphodiesterase 4D, 4-[8-(3-nitrophenyl)-1,7-naphthyridin-6-yl]benzoic acid, ZINC ION, ... (5 entities in total)
• 機能のキーワード: pde4d selective inhibitor nvp, alternative splicing, camp, cytoplasm, cytoskeleton, hydrolase, membrane, metal-binding, phosphorylation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm (By similarity): Q08499
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76509.73

構造登録者

Ke, H. (登録日: 2007-08-15, 公開日: 2008-04-08, 最終更新日: 2024-04-03)

主引用文献

Wang, H.,Peng, M.S.,Chen, Y.,Geng, J.,Robinson, H.,Houslay, M.D.,Cai, J.,Ke, H.
Structures of the four subfamilies of phosphodiesterase-4 provide insight into the selectivity of their inhibitors.
Biochem.J., 408:193-201, 2007

PubMed Abstract: PDE4 (phosphodiesterase-4)-selective inhibitors have attracted much attention as potential therapeutics for the treatment of both depression and major inflammatory diseases, but their practical application has been compromised by side effects. A possible cause for the side effects is that current PDE4-selective inhibitors similarly inhibit isoforms from all four PDE4 subfamilies. The development of PDE4 subfamily-selective inhibitors has been hampered by a lack of structural information. In the present study, we rectify this by providing the crystal structures of the catalytic domains of PDE4A, PDE4B and PDE4D in complex with the PDE4 inhibitor NVP {4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid} as well as the unliganded PDE4C structure. NVP binds in the same conformation to the deep cAMP substrate pocket and interacts with the same residues in each instance. However, detailed structural comparison reveals significant conformational differences. Although the active sites of PDE4B and PDE4D are mostly comparable, PDE4A shows significant displacements of the residues next to the invariant glutamine residue that is critical for substrate and inhibitor binding. PDE4C appears to be more distal from other PDE4 subfamilies, with certain key residues being disordered. Our analyses provide the first structural basis for the development of PDE4 subfamily-selective inhibitors.

PubMed: 17727341
DOI: 10.1042/BJ20070970

実験手法

X-RAY DIFFRACTION (1.57 Å)