2QVN

Crystal structure of adenosine deaminase from Plasmodium vivax in complex with guanosine

2QVN の概要

• エントリーDOI: 10.2210/pdb2qvn/pdb
• 関連するPDBエントリー: 2PGF 2PGR
• 分子名称: Adenosine deaminase, GUANOSINE, 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: metallo-dependent hydrolase, tim barrel, structural genomics, medical structural genomics of pathogenic protozoa consortium, msgpp, plasmodium, malaria, psi, protein structure initiative, hydrolase
• 由来する生物種: Plasmodium vivax SaI-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43967.71

構造登録者

Larson, E.T.,Merritt, E.A.,Structural Genomics of Pathogenic Protozoa Consortium (SGPP) (登録日: 2007-08-08, 公開日: 2007-09-04, 最終更新日: 2024-11-13)

主引用文献

Larson, E.T.,Deng, W.,Krumm, B.E.,Napuli, A.,Mueller, N.,Van Voorhis, W.C.,Buckner, F.S.,Fan, E.,Lauricella, A.,DeTitta, G.,Luft, J.,Zucker, F.,Hol, W.G.,Verlinde, C.L.,Merritt, E.A.
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity.
J.Mol.Biol., 381:975-988, 2008

PubMed Abstract: Plasmodium and other apicomplexan parasites are deficient in purine biosynthesis, relying instead on the salvage of purines from their host environment. Therefore, interference with the purine salvage pathway is an attractive therapeutic target. The plasmodial enzyme adenosine deaminase (ADA) plays a central role in purine salvage and, unlike mammalian ADA homologs, has a further secondary role in methylthiopurine recycling. For this reason, plasmodial ADA accepts a wider range of substrates, as it is responsible for deamination of both adenosine and 5'-methylthioadenosine. The latter substrate is not accepted by mammalian ADA homologs. The structural basis for this natural difference in specificity between plasmodial and mammalian ADA has not been well understood. We now report crystal structures of Plasmodium vivax ADA in complex with adenosine, guanosine, and the picomolar inhibitor 2'-deoxycoformycin. These structures highlight a drastic conformational change in plasmodial ADA upon substrate binding that has not been observed for mammalian ADA enzymes. Further, these complexes illuminate the structural basis for the differential substrate specificity and potential drug selectivity between mammalian and parasite enzymes.

PubMed: 18602399
DOI: 10.1016/j.jmb.2008.06.048

実験手法

X-RAY DIFFRACTION (2.19 Å)