2QUW

Hammerhead Ribozyme G12A mutant after cleavage

2QUW の概要

• エントリーDOI: 10.2210/pdb2quw/pdb
• 関連するPDBエントリー: 2GOZ 2QUS
• 分子名称: Hammerhead ribozyme, cleaved fragment, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: hammerhead ribozyme, ribozyme, catalytic rna, ribozyme-product complex, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45255.53

構造登録者

Chi, Y.I.,Scott, W.G.,Kim, S.H. (登録日: 2007-08-06, 公開日: 2008-07-15, 最終更新日: 2024-02-21)

主引用文献

Chi, Y.I.,Martick, M.,Lares, M.,Kim, R.,Scott, W.G.,Kim, S.H.
Capturing hammerhead ribozyme structures in action by modulating general base catalysis.
Plos Biol., 6:e234-e234, 2008

PubMed Abstract: We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures.

PubMed: 18834200
DOI: 10.1371/journal.pbio.0060234

実験手法

X-RAY DIFFRACTION (2.2 Å)