2QUS

Hammerhead Ribozyme G12A mutant pre-cleavage

Summary for 2QUS

• Entry DOI: 10.2210/pdb2qus/pdb
• Related: 2GOZ 2QUS 2QUW
• Descriptor: Hammerhead ribozyme, MAGNESIUM ION (3 entities in total)
• Functional Keywords: hammerhead rna, hammerhead ribozyme, catalytic rna, satellite virus rna, ribozyme, rna
• Total number of polymer chains: 2
• Total formula weight: 45197.21

Authors

Chi, Y.I.,Scott, W.G.,Kim, S.H. (deposition date: 2007-08-06, release date: 2008-07-15, Last modification date: 2024-04-03)

Primary citation

Chi, Y.I.,Martick, M.,Lares, M.,Kim, R.,Scott, W.G.,Kim, S.H.
Capturing hammerhead ribozyme structures in action by modulating general base catalysis.
Plos Biol., 6:e234-e234, 2008

PubMed Abstract: We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures.

PubMed: 18834200
DOI: 10.1371/journal.pbio.0060234

Experimental method

X-RAY DIFFRACTION (2.4 Å)