2QUS
Hammerhead Ribozyme G12A mutant pre-cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.926, 53.030, 71.963 |
| Unit cell angles | 74.57, 81.37, 75.61 |
Refinement procedure
| Resolution | 25.670 - 2.400 |
| R-factor | 0.191 |
| Rwork | 0.184 |
| R-free | 0.25300 |
| Starting model (for MR) | small helical fragments |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.850 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.670 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.054 |
| Number of reflections | 11632 |
| <I/σ(I)> | 8 |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
