2QOG

Crotoxin B, the basic PLA2 from Crotalus durissus terrificus.

2QOG の概要

• エントリーDOI: 10.2210/pdb2qog/pdb
• 関連するPDBエントリー: 2OK9 2OQD
• 分子名称: Phospholipase A2 CB2, Phospholipase A2 CB1, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: crotoxin b cd-cdt basic-pla2, calcium, hydrolase, lipid degradation, metal-binding, secreted, neurotoxin, presynaptic neurotoxin
• 由来する生物種: Crotalus durissus terrificus (tropical rattlesnake); 詳細
• 細胞内の位置: Secreted: P24027 P62022
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57083.85

構造登録者

Marchi-Salvador, D.P.,Correa, L.C.,Fontes, M.R.M. (登録日: 2007-07-20, 公開日: 2008-04-01, 最終更新日: 2024-11-20)

主引用文献

Marchi-Salvador, D.P.,Correa, L.C.,Magro, A.J.,Oliveira, C.Z.,Soares, A.M.,Fontes, M.R.
Insights into the role of oligomeric state on the biological activities of crotoxin: crystal structure of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom.
Proteins, 72:883-891, 2008

PubMed Abstract: Crotoxin B (CB or Cdt PLA(2)) is a basic Asp49-PLA(2) found in the venom of Crotalus durissus terrificus and it is one of the subunits that constitute the crotoxin (Cro). This heterodimeric toxin, main component of the C. d. terrificus venom, is completed by an acidic, nontoxic, and nonenzymatic component (crotoxin A, CA or crotapotin), and it is related to important envenomation effects such as neurological disorders, myotoxicity, and renal failure. Although Cro has been crystallized since 1938, no crystal structure of this toxin or its subunits is currently available. In this work, the authors present the crystal structure of a novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2). The results suggest that these assemblies are stable in solution and show that Ser1 and Glu92 of CB1 and CB2, respectively, play an important role in the oligomerization. The tetrameric and dimeric conformations resulting from the association of the isoforms may increase the neurotoxicity of the toxin CB by the creation of new binding sites, which could improve the affinity of the molecular complexes to the presynaptic membrane.

PubMed: 18275084
DOI: 10.1002/prot.21980

実験手法

X-RAY DIFFRACTION (2.28 Å)