2QNO

Crystal Structure of the Mutant E55Q of the Cellulase CEL48F in Complex with a Thio-Oligosaccharide

Summary for 2QNO

Entry DOI	10.2210/pdb2qno/pdb
Related	1g9g 1g9j
Descriptor	Endoglucanase F, 4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
Functional Keywords	cellulase, thio-oligosaccharide complex, inactive mutant, gh family 48, alpha-alpha-six barrel, carbohydrate metabolism, cellulose degradation, glycosidase, hydrolase, polysaccharide degradation
Biological source	Clostridium cellulolyticum
Total number of polymer chains	1
Total formula weight	72466.58
Authors	Parsiegla, G.,Haser, R. (deposition date: 2007-07-19, release date: 2008-01-15, Last modification date: 2023-08-30)
Primary citation	Parsiegla, G.,Reverbel, C.,Tardif, C.,Driguez, H.,Haser, R. Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action J.Mol.Biol., 375:499-510, 2008 Cited by PubMed: 18035374 DOI: 10.1016/j.jmb.2007.10.039 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation