2QFI

Structure of the zinc transporter YiiP

2QFI の概要

• エントリーDOI: 10.2210/pdb2qfi/pdb
• 分子名称: Ferrous-iron efflux pump fieF, ZINC ION (2 entities in total)
• 機能のキーワード: zinc transporter, transport protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P69380
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66372.19

構造登録者

Lu, M. (登録日: 2007-06-27, 公開日: 2007-10-16, 最終更新日: 2024-02-21)

主引用文献

Lu, M.,Fu, D.
Structure of the zinc transporter YiiP.
Science, 317:1746-1748, 2007

PubMed Abstract: YiiP is a membrane transporter that catalyzes Zn2+/H+ exchange across the inner membrane of Escherichia coli. Mammalian homologs of YiiP play critical roles in zinc homeostasis and cell signaling. Here, we report the x-ray structure of YiiP in complex with zinc at 3.8 angstrom resolution. YiiP is a homodimer held together in a parallel orientation through four Zn2+ ions at the interface of the cytoplasmic domains, whereas the two transmembrane domains swing out to yield a Y-shaped structure. In each protomer, the cytoplasmic domain adopts a metallochaperone-like protein fold; the transmembrane domain features a bundle of six transmembrane helices and a tetrahedral Zn2+ binding site located in a cavity that is open to both the membrane outer leaflet and the periplasm.

PubMed: 17717154
DOI: 10.1126/science.1143748

実験手法

X-RAY DIFFRACTION (3.8 Å)