2QFB

Crystal structure of the regulatory domain of human RIG-I with bound Zn

2QFB の概要

• エントリーDOI: 10.2210/pdb2qfb/pdb
• 関連するPDBエントリー: 2QFD
• 分子名称: Probable ATP-dependent RNA helicase DDX58, ZINC ION (3 entities in total)
• 機能のキーワード: zinc finger, alternative splicing, antiviral defense, atp-binding, helicase, hydrolase, immune response, innate immunity, interferon induction, nucleotide-binding, polymorphism, rna-binding, ubl conjugation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: O95786
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 169189.07

構造登録者

Cui, S.,Lammens, A.,Lammens, K.,Hopfner, K.P. (登録日: 2007-06-27, 公開日: 2008-02-12, 最終更新日: 2024-11-20)

主引用文献

Cui, S.,Eisenacher, K.,Kirchhofer, A.,Brzozka, K.,Lammens, A.,Lammens, K.,Fujita, T.,Conzelmann, K.K.,Krug, A.,Hopfner, K.P.
The C-Terminal Regulatory Domain Is the RNA 5'-Triphosphate Sensor of RIG-I.
Mol.Cell, 29:169-179, 2008

PubMed Abstract: The ATPase RIG-I senses viral RNAs that contain 5'-triphosphates in the cytoplasm. It initiates a signaling cascade that activates innate immune response by interferon and cytokine production, providing essential antiviral protection for the host. The mode of RNA 5'-triphosphate sensing by RIG-I remains elusive. We show that the C-terminal regulatory domain RD of RIG-I binds viral RNA in a 5'-triphosphate-dependent manner and activates the RIG-I ATPase by RNA-dependent dimerization. The crystal structure of RD reveals a zinc-binding domain that is structurally related to GDP/GTP exchange factors of Rab-like GTPases. The zinc coordination site is essential for RIG-I signaling and is also conserved in MDA5 and LGP2, suggesting related RD domains in all three enzymes. Structure-guided mutagenesis identifies a positively charged groove as likely 5'-triphosphate-binding site of RIG-I. This groove is distinct in MDA5 and LGP2, raising the possibility that RD confers ligand specificity.

PubMed: 18243112
DOI: 10.1016/j.molcel.2007.10.032

実験手法

X-RAY DIFFRACTION (3 Å)