2Q86

Structure of the mouse invariant NKT cell receptor Valpha14

Summary for 2Q86

• Entry DOI: 10.2210/pdb2q86/pdb
• Related: 2CDE 2EYR
• Descriptor: Valpha14 TCR, Vbeta8.2, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: inkt cells, tcr, glycolipid recognition, innate immunity, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 108793.76

Authors

Zajonc, D.M. (deposition date: 2007-06-08, release date: 2008-04-01, Last modification date: 2024-11-06)

Primary citation

Zajonc, D.M.,Savage, P.B.,Bendelac, A.,Wilson, I.A.,Teyton, L.
Crystal Structures of Mouse CD1d-iGb3 Complex and its Cognate Valpha14 T Cell Receptor Suggest a Model for Dual Recognition of Foreign and Self Glycolipids.
J.Mol.Biol., 377:1104-1116, 2008

PubMed Abstract: The semi-invariant Valpha14Jalpha18 T cell receptor (TCR) is expressed by regulatory NKT cells and has the unique ability to recognize chemically diverse ligands presented by CD1d. The crystal structure of CD1d complexed to a natural, endogenous ligand, isoglobotrihexosylceramide (iGb3), illustrates the extent of this diversity when compared to the binding of potent, exogenous ligands, such as alpha-galactosylceramide (alpha-GalCer). A single mode of recognition for these two classes of ligands would then appear problematic for a single T cell receptor. However, the Valpha14 TCR adopts two different conformations in the crystal where, in one configuration, the presence of a larger cavity between the two CDR3 regions could accommodate iGb3 and, in the other, a smaller cavity fits alpha-GalCer more snugly. Alternatively, the extended iGb3 headgroup could be "squashed" upon docking of the TCR and accommodated between the CD1 and TCR surfaces. Thus, the same TCR may adopt alternative modes of recognition for these foreign and self-ligands for NKT cell activation.

PubMed: 18295796
DOI: 10.1016/j.jmb.2008.01.061

Experimental method

X-RAY DIFFRACTION (1.85 Å)