2PXC

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAM and GTPA

2PXC の概要

• エントリーDOI: 10.2210/pdb2pxc/pdb
• 関連するPDBエントリー: 2PX2 2PX4 2PX5 2PX8 2PXA
• 分子名称: Genome polyprotein [Contains: Capsid protein C (Core protein); Envelope protein M (Matrix protein); Major envelope protein E; Non-structural protein 1 (NS1); Non-structural protein 2A (NS2A); Flavivirin protease NS2B regulatory subunit; Flavivirin protease NS3 catalytic subunit; Non-structural protein 4A (NS4A); Non-structural protein 4B (NS4B); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5)], S-ADENOSYLMETHIONINE, GUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: murray valley encephalitis virus, methyltransferase, sam, gtpa, g3a, structural genomics, oxford protein production facility, oppf, transferase
• 由来する生物種: Murray valley encephalitis virus (strain MVE-1-51)
• 細胞内の位置: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P05769
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32250.10

構造登録者

Assenberg, R.,Ren, J.,Verma, A.,Walter, T.S.,Alderton, D.,Hurrelbrink, R.J.,Fuller, S.D.,Owens, R.J.,Stuart, D.I.,Grimes, J.M.,Oxford Protein Production Facility (OPPF) (登録日: 2007-05-14, 公開日: 2007-05-29, 最終更新日: 2023-08-30)

主引用文献

Assenberg, R.,Ren, J.,Verma, A.,Walter, T.S.,Alderton, D.,Hurrelbrink, R.J.,Fuller, S.D.,Bressanelli, S.,Owens, R.J.,Stuart, D.I.,Grimes, J.M.
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues.
J.Gen.Virol., 88:2228-2236, 2007

PubMed Abstract: We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

PubMed: 17622627
DOI: 10.1099/vir.0.82757-0

実験手法

X-RAY DIFFRACTION (2.8 Å)