2PUF

CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES

Summary for 2PUF

• Entry DOI: 10.2210/pdb2puf/pdb
• Descriptor: DNA (5'-D(*TP*AP*CP*GP*CP*AP*AP*AP*CP*GP*TP*TP*TP*GP*CP*GP*T )-3'), PROTEIN (PURINE REPRESSOR), GUANINE, ... (4 entities in total)
• Functional Keywords: complex (dna-binding protein-dna), dna-binding regulatory protein, extended corepressor specificity, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 43416.04

Authors

Lu, F.,Schumacher, M.A.,Arvidson, D.N.,Haldimann, A.,Wanner, B.L.,Zalkin, H.,Brennan, R.G. (deposition date: 1997-10-04, release date: 1998-05-06, Last modification date: 2024-02-21)

• Primary citation: Lu, F.,Schumacher, M.A.,Arvidson, D.N.,Haldimann, A.,Wanner, B.L.,Zalkin, H.,Brennan, R.G.; Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190.; Biochemistry, 37:971-982, 1998; PubMed: 9454587; DOI: 10.1021/bi971942s

Experimental method

X-RAY DIFFRACTION (3 Å)