2PLL

Crystal structure of perdeuterated human arginase I

Summary for 2PLL

• Entry DOI: 10.2210/pdb2pll/pdb
• Related: 2AEB
• Descriptor: arginase-1, MANGANESE (II) ION, 2(S)-AMINO-6-BORONOHEXANOIC ACID, ... (4 entities in total)
• Functional Keywords: perdeuterated protein; x-ray structure, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P05089
• Total number of polymer chains: 2
• Total formula weight: 70163.51

Authors

Di Costanzo, L.,Moulin, M.,Haertlein, M.,Meilleur, F.,Christianson, D.W. (deposition date: 2007-04-19, release date: 2007-08-14, Last modification date: 2023-08-30)

Primary citation

Di Costanzo, L.,Moulin, M.,Haertlein, M.,Meilleur, F.,Christianson, D.W.
Expression, purification, assay, and crystal structure of perdeuterated human arginase I
Arch.Biochem.Biophys., 465:82-89, 2007

PubMed Abstract: Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of l-arginine to yield l-ornithine and urea. In order to establish a foundation for future neutron diffraction studies that will provide conclusive structural information regarding proton/deuteron positions in enzyme-inhibitor complexes, we have expressed, purified, assayed, and determined the X-ray crystal structure of perdeuterated (i.e., fully deuterated) human arginase I complexed with 2(S)-amino-6-boronohexanoic acid (ABH) at 1.90A resolution. Prior to the neutron diffraction experiment, it is important to establish that perdeuteration does not cause any unanticipated structural or functional changes. Accordingly, we find that perdeuterated human arginase I exhibits catalytic activity essentially identical to that of the unlabeled enzyme. Additionally, the structure of the perdeuterated human arginase I-ABH complex is identical to that of the corresponding complex with the unlabeled enzyme. Therefore, we conclude that crystals of the perdeuterated human arginase I-ABH complex are suitable for neutron crystallographic study.

PubMed: 17562323
DOI: 10.1016/j.abb.2007.04.036

Experimental method

X-RAY DIFFRACTION (1.9 Å)