2PG1

Structural analysis of a cytoplasmic dynein Light Chain-Intermediate Chain complex

2PG1 の概要

• エントリーDOI: 10.2210/pdb2pg1/pdb
• 分子名称: Dynein light chain 1, cytoplasmic, Dynein light chain Tctex-type, Cytoplasmic dynein 1 intermediate chain 2, ... (5 entities in total)
• 機能のキーワード: dynein intermediate chain, dynein light chain, lc8, pin, tctex1, structural protein
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: Q24117 Q62871; Cytoplasm, cytoskeleton (Probable): Q94524
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 108256.85

構造登録者

Williams, J.C.,Hendrickson, W.A. (登録日: 2007-04-06, 公開日: 2007-06-05, 最終更新日: 2024-10-16)

主引用文献

Williams, J.C.,Roulhac, P.L.,Roy, A.G.,Vallee, R.B.,Fitzgerald, M.C.,Hendrickson, W.A.
Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex
Proc.Natl.Acad.Sci.Usa, 104:10028-10033, 2007

PubMed Abstract: Cytoplasmic dynein is a microtubule-based motor protein complex that plays important roles in a wide range of fundamental cellular processes, including vesicular transport, mitosis, and cell migration. A single major form of cytoplasmic dynein associates with membranous organelles, mitotic kinetochores, the mitotic and migratory cell cortex, centrosomes, and mRNA complexes. The ability of cytoplasmic dynein to recognize such diverse forms of cargo is thought to be associated with its several accessory subunits, which reside at the base of the molecule. The dynein light chains (LCs) LC8 and TcTex1 form a subcomplex with dynein intermediate chains, and they also interact with numerous protein and ribonucleoprotein partners. This observation has led to the hypothesis that these subunits serve to tether cargo to the dynein motor. Here, we present the structure and a thermodynamic analysis of a complex of LC8 and TcTex1 associated with their intermediate chain scaffold. The intermediate chains effectively block the major putative cargo binding sites within the light chains. These data suggest that, in the dynein complex, the LCs do not bind cargo, in apparent disagreement with a role for LCs in dynein cargo binding interactions.

PubMed: 17551010
DOI: 10.1073/pnas.0703614104

実験手法

X-RAY DIFFRACTION (2.8 Å)