2PCF

THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

Summary for 2PCF

• Entry DOI: 10.2210/pdb2pcf/pdb
• Descriptor: PLASTOCYANIN, CYTOCHROME F, COPPER (II) ION, ... (4 entities in total)
• Functional Keywords: electron transport, paramagnetic, chemical shift, complex formation, dynamic complex, photosynthesis, pseudocontact shift, complex (electron transport proteins)
• Biological source: Spinacia oleracea (spinach); More
• Cellular location: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00289; Plastid, chloroplast thylakoid membrane; Single-pass membrane protein: P36438
• Total number of polymer chains: 2
• Total formula weight: 38420.79

Authors

Ubbink, M.,Ejdeback, M.,Karlsson, B.G.,Bendall, D.S. (deposition date: 1997-12-22, release date: 1998-04-08, Last modification date: 2024-10-23)

Primary citation

Ubbink, M.,Ejdeback, M.,Karlsson, B.G.,Bendall, D.S.
The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics.
Structure, 6:323-335, 1998

PubMed Abstract: The reduction of plastocyanin by cytochrome f is part of the chain of photosynthetic electron transfer reactions that links photosystems II and I. The reaction is rapid and is influenced by charged residues on both proteins. Previously determined structures show that the plastocyanin copper and cytochrome f haem redox centres are some distance apart from the relevant charged sidechains, and until now it was unclear how a transient electrostatic complex can be formed that brings the redox centres sufficiently close for a rapid reaction.

PubMed: 9551554
DOI: 10.1016/S0969-2126(98)00035-5

Experimental method

SOLUTION NMR