2P6T
CRYSTAL STRUCTURE OF TRANSCRIPTIONAL REGULATOR NMB0573 and L-LEUCINE COMPLEX FROM NEISSERIA MENINGITIDIS
Summary for 2P6T
| Entry DOI | 10.2210/pdb2p6t/pdb |
| Descriptor | Transcriptional regulator, LRP/AsnC family, LEUCINE, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | nmb0573, transcriptional regulator, lrp/asnc-family, n. meninigitidis, structural genomics, structural and functional analysis of n. meninigitidis transcriptional regulators, oxford protein production facility, oppf, transcription |
| Biological source | Neisseria meningitidis |
| Total number of polymer chains | 8 |
| Total formula weight | 148352.41 |
| Authors | Ren, J.,Sainsbury, S.,Owens, R.J.,Oxford Protein Production Facility (OPPF) (deposition date: 2007-03-19, release date: 2007-04-03, Last modification date: 2024-10-16) |
| Primary citation | Ren, J.,Sainsbury, S.,Combs, S.E.,Capper, R.G.,Jordan, P.W.,Berrow, N.S.,Stammers, D.K.,Saunders, N.J.,Owens, R.J. The Structure and Transcriptional Analysis of a Global Regulator from Neisseria meningitidis. J.Biol.Chem., 282:14655-14664, 2007 Cited by PubMed Abstract: Neisseria meningitidis, a causative agent of bacterial meningitis, has a relatively small repertoire of transcription factors, including NMB0573 (annotated AsnC), a member of the Lrp-AsnC family of regulators that are widely expressed in both Bacteria and Archaea. In the present study we show that NMB0573 binds to l-leucine and l-methionine and have solved the structure of the protein with and without bound amino acids. This has shown, for the first time that amino acid binding does not induce significant conformational changes in the structure of an AsnC/Lrp regulator although it does appear to stabilize the octameric assembly of the protein. Transcriptional profiling of wild-type and NMB0573 knock-out strains of N. meningitidis has shown that NMB0573 is associated with an adaptive response to nutrient poor conditions reflected in a reduction in major surface protein expression. On the basis of its structure and the transcriptional response, we propose that NMB0573 is a global regulator in Neisseria controlling responses to nutrient availability through indicators of general amino acid abundance: leucine and methionine. PubMed: 17374605DOI: 10.1074/jbc.M701082200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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