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2P48

Complex of a camelid single-domain vhh antibody fragment with RNASE A at 2.3A resolution: SE5B-tetra crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.

Summary for 2P48
Entry DOI10.2210/pdb2p48/pdb
DescriptorRibonuclease pancreatic, ANTIBODY CAB-RN05, SULFATE ION, ... (4 entities in total)
Functional Keywordssemet phasing, camelid single-domain antibody, vhh, cab-rn05, rnase a, yeast surface display., hydrolase, hydrolase-immune system complex, hydrolase/immune system
Biological sourceCamelus dromedarius (Arabian camel)
More
Cellular locationSecreted: P61823
Total number of polymer chains2
Total formula weight27241.45
Authors
Tereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A. (deposition date: 2007-03-11, release date: 2008-03-11, Last modification date: 2023-11-15)
Primary citationTereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A.
Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold
Protein Sci., 17:1175-1187, 2008
Cited by
PubMed: 18445622
DOI: 10.1110/ps.034892.108
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.3 Å)
Structure validation

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