2OXV
Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.
Summary for 2OXV
| Entry DOI | 10.2210/pdb2oxv/pdb |
| Descriptor | DNA (5'-D(*TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'), Type II restriction enzyme EcoRI (3 entities in total) |
| Functional Keywords | ecori, type ii restriction endonuclease, protein-dna interactions, promiscuous mutant, relaxed specificity mutant, hydrolase-dna complex, hydrolase/dna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 2 |
| Total formula weight | 35098.99 |
| Authors | Sapienza, P.J.,Rosenberg, J.M.,Jen-Jacobson, L. (deposition date: 2007-02-21, release date: 2007-10-23, Last modification date: 2023-08-30) |
| Primary citation | Sapienza, P.J.,Rosenberg, J.M.,Jen-Jacobson, L. Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease. Structure, 15:1368-1382, 2007 Cited by PubMed: 17997963DOI: 10.1016/j.str.2007.09.014 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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