2OTL

Girodazole bound to the large subunit of Haloarcula marismortui

Summary for 2OTL

• Entry DOI: 10.2210/pdb2otl/pdb
• Related: 2OTJ
• Descriptor: 23S ribosomal RNA, 50S ribosomal protein L10e, 50S ribosomal protein L13P, ... (38 entities in total)
• Functional Keywords: girodazole, girolline, antibiotic complex, 50s, ribosome, large subunit
• Biological source: Haloarcula marismortui; More
• Cellular location: Cytoplasm : P12743
• Total number of polymer chains: 31
• Total formula weight: 1478307.14

Authors

Blaha, G.,Schroeder, S.J.,Tirado-Rives, J. (deposition date: 2007-02-08, release date: 2007-04-03, Last modification date: 2024-11-13)

Primary citation

Schroeder, S.J.,Blaha, G.,Tirado-Rives, J.,Steitz, T.A.,Moore, P.B.
The Structures of Antibiotics Bound to the E Site Region of the 50 S Ribosomal Subunit of Haloarcula marismortui: 13-Deoxytedanolide and Girodazole.
J.Mol.Biol., 367:1471-1479, 2007

PubMed Abstract: Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria.

PubMed: 17321546
DOI: 10.1016/j.jmb.2007.01.081

Experimental method

X-RAY DIFFRACTION (2.7 Å)