2OO4

Structure of LNR-HD (Negative Regulatory Region) from human Notch 2

2OO4 の概要

• エントリーDOI: 10.2210/pdb2oo4/pdb
• 分子名称: Neurogenic locus notch homolog protein 2, CALCIUM ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: alpha-beta-sandwich; sea domain; lnr; lin12 notch repeat; cysteine-rich; hd domain, cell cycle, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Notch 2 extracellular truncation: Cell membrane ; Single-pass type I membrane protein . Notch 2 intracellular domain: Nucleus : Q04721
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52972.31

構造登録者

Gordon, W.R.,Vardar-Ulu, D.,Histen, G.,Sanchez-Irizarry, C.,Aster, J.C.,Blacklow, S.C. (登録日: 2007-01-25, 公開日: 2007-04-03, 最終更新日: 2024-11-20)

主引用文献

Gordon, W.R.,Vardar-Ulu, D.,Histen, G.,Sanchez-Irizarry, C.,Aster, J.C.,Blacklow, S.C.
Structural basis for autoinhibition of Notch
Nat.Struct.Mol.Biol., 14:295-300, 2007

PubMed Abstract: Notch receptors transmit signals between adjacent cells. Signaling is initiated when ligand binding induces metalloprotease cleavage of Notch within an extracellular negative regulatory region (NRR). We present here the X-ray structure of the human NOTCH2 NRR, which adopts an autoinhibited conformation. Extensive interdomain interactions within the NRR bury the metalloprotease site, showing that a substantial conformational movement is necessary to expose this site during activation by ligand. Leukemia-associated mutations in NOTCH1 probably release autoinhibition by destabilizing the conserved hydrophobic core of the NRR.

PubMed: 17401372
DOI: 10.1038/nsmb1227

実験手法

X-RAY DIFFRACTION (2.003 Å)