2ONH
Crystal Structure of of limonene synthase with 2-fluorolinalyl diphosphate(FLPP)
Summary for 2ONH
| Entry DOI | 10.2210/pdb2onh/pdb |
| Descriptor | 4S-limonene synthase, MANGANESE (II) ION, (1S)-1-[(1S)-1-FLUOROETHYL]-1,5-DIMETHYLHEXYL TRIHYDROGEN DIPHOSPHATE, ... (5 entities in total) |
| Functional Keywords | monoterpene synthase, monoterpene cyclase, geranyl diphosphate, 2 fluorogeranyl diphosphate, linalyl diphosphate, 2-fluorolinalyl diphosphate, lyase |
| Biological source | Mentha spicata (spearmint) |
| Total number of polymer chains | 2 |
| Total formula weight | 129001.48 |
| Authors | Hyatt, D.C.,Youn, B.,Croteau, R.,Kang, C. (deposition date: 2007-01-24, release date: 2007-03-06, Last modification date: 2023-08-30) |
| Primary citation | Hyatt, D.C.,Youn, B.,Zhao, Y.,Santhamma, B.,Coates, R.M.,Croteau, R.B.,Kang, C. Structure of limonene synthase, a simple model for terpenoid cyclase catalysis. Proc.Natl.Acad.Sci.Usa, 104:5360-5365, 2007 Cited by PubMed Abstract: The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction. PubMed: 17372193DOI: 10.1073/pnas.0700915104 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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