2OLC

Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho

2OLC の概要

• エントリーDOI: 10.2210/pdb2olc/pdb
• 分子名称: Methylthioribose kinase, HOLMIUM ATOM, 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE, ... (5 entities in total)
• 機能のキーワード: kinase adp-2ho complex, transferase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92408.94

構造登録者

Ku, S.Y.,Smith, G.D.,Howell, P.L. (登録日: 2007-01-18, 公開日: 2007-05-22, 最終更新日: 2023-12-27)

主引用文献

Ku, S.Y.,Smith, G.D.,Howell, P.L.
ADP-2Ho as a phasing tool for nucleotide-containing proteins.
Acta Crystallogr.,Sect.D, 63:493-499, 2007

PubMed Abstract: Trivalent holmium ions were shown to isomorphously replace magnesium ions to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex provided sufficient phasing power to allow SAD and SIRAS phasing of this previously unknown structure using the L(III) absorption edge of holmium. The structure of ADP-2Ho reveals that the two Ho ions are approximately 4 A apart and are likely to share their ligands: the phosphoryl O atoms of ADP and a water molecule. The structure determination of MTR kinase using data collected using Cu Kalpha X-radiation was also attempted. Although the heavy-atom substructure determination was successful, interpretation of the map was more challenging. The isomorphous substitution of holmium for magnesium in the MTR kinase-nucleotide complex suggests that this could be a useful phasing tool for other metal-dependent nucleotide-containing proteins.

PubMed: 17372354
DOI: 10.1107/S0907444907006592

実験手法

X-RAY DIFFRACTION (2 Å)