AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

Summary for 2O9L

Descriptor5'-D(*TP*TP*TP*TP*GP*GP*CP*AP*CP*GP*TP*TP*TP*C)-3', 5'-D(*GP*AP*AP*AP*CP*GP*TP*GP*CP*CP*AP*AP*AP*A)-3', RNA polymerase sigma factor RpoN (3 entities in total)
Functional Keywordsamber, generalized born solvent model, protein-dna complex, helix-turn-helix, transcription factor, sigma-54, rna polymerase, rna polymerase sigma factor rpon-dna complex, rna polymerase sigma factor rpon/dna
Biological sourceAquifex aeolicus
Total number of polymer chains3
Total molecular weight16048.41
Doucleff, M.,Pelton, J.G.,Lee, P.S.,Wemmer, D.E. (deposition date: 2006-12-13, release date: 2007-07-17, Last modification date: 2011-07-13)
Primary citation
Doucleff, M.,Pelton, J.G.,Lee, P.S.,Nixon, B.T.,Wemmer, D.E.
Structural basis of DNA recognition by the alternative sigma-factor, sigma54.
J.Mol.Biol., 369:1070-1078, 2007
PubMed: 17481658 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2007.04.019
MImport into Mendeley
Experimental method
NMR Information

Structure validation

ClashscoreRamachandran outliersSidechain outliers1 7.6% 10.7%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures
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PDB entries from 2020-09-23