2O90

Atomic resolution crystal structure of E.coli dihydroneopterin aldolase in complex with neopterin

2O90 の概要

• エントリーDOI: 10.2210/pdb2o90/pdb
• 関連するPDBエントリー: 1DHN 2DHN 2NM2 2NM3 2O9M 2OAK
• 分子名称: Dihydroneopterin aldolase, L-NEOPTERIN (3 entities in total)
• 機能のキーワード: dihydroneopterin aldolase, dhna, neopterin, monapterin, 7, 8-dihydroneopterin, drug design, atomic resolution, lyase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13886.69

構造登録者

Blaszczyk, J.,Ji, X.,Yan, H. (登録日: 2006-12-12, 公開日: 2007-12-25, 最終更新日: 2023-08-30)

主引用文献

Blaszczyk, J.,Lu, Z.,Li, Y.,Yan, H.,Ji, X.
Crystallographic and molecular dynamics simulation analysis of Escherichia coli dihydroneopterin aldolase.
Cell Biosci, 4:52-52, 2014

PubMed Abstract: Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin and also the epimerization of DHNP to 7,8-dihydromonapterin. Previously, we determined the crystal structure of Staphylococcus aureus DHNA (SaDHNA) in complex with the substrate analogue neopterin (NP). We also showed that Escherichia coli DHNA (EcDHNA) and SaDHNA have significantly different binding and catalytic properties by biochemical analysis. On the basis of these structural and functional data, we proposed a catalytic mechanism involving two proton wires.

PubMed: 25264482
DOI: 10.1186/2045-3701-4-52

実験手法

X-RAY DIFFRACTION (1.07 Å)