2O20

Crystal structure of transcription regulator CcpA of Lactococcus lactis

2O20 の概要

• エントリーDOI: 10.2210/pdb2o20/pdb
• 関連するPDBエントリー: 1RZR 1SHI 1SXG 1SXH 2FEP 2HSG
• 分子名称: Catabolite control protein A, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: ccpa, transcriptional regulator, helix-turn-helix, transcription
• 由来する生物種: Lactococcus lactis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 293944.86

構造登録者

Loll, B.,Kowalczyk, M.,Alings, C.,Chieduch, A.,Bardowski, J.,Saenger, W.,Biesiadka, J. (登録日: 2006-11-29, 公開日: 2007-03-27, 最終更新日: 2023-08-30)

主引用文献

Loll, B.,Kowalczyk, M.,Alings, C.,Chieduch, A.,Bardowski, J.,Saenger, W.,Biesiadka, J.
Structure of the transcription regulator CcpA from Lactococcus lactis
Acta Crystallogr.,Sect.D, 63:431-436, 2007

PubMed Abstract: Catabolite control protein A (CcpA) functions as master transcriptional regulator of carbon catabolism in Firmicutes. It belongs to the family of bacterial repressor/regulator proteins. Here, the crystal structure of the 76 kDa homodimeric CcpA protein from Lactococcus lactis subsp. lactis IL1403 is presented at 1.9 A resolution in the absence of cognate DNA. The phases were derived by molecular replacement and the structure was refined to crystallographic R and R(free) factors of 0.177 and 0.211, respectively. The presence of a sulfate molecule in the direct vicinity of a putative effector-binding site in the monomer allowed the derivation of a model for the possible binding of small organic effector molecules.

PubMed: 17372346
DOI: 10.1107/S0907444907000546

実験手法

X-RAY DIFFRACTION (1.9 Å)