2NY4
HIV-1 gp120 Envelope Glycoprotein (K231C, T257S, E268C, S334A, S375W) Complexed with CD4 and Antibody 17b
Summary for 2NY4
| Entry DOI | 10.2210/pdb2ny4/pdb |
| Related | 1GC1 1RZJ 1RZK 2NXY 2NXZ 2NY0 2NY1 2NY2 2NY3 2NY5 2NY6 2NY7 |
| Related PRD ID | PRD_900003 |
| Descriptor | ENVELOPE GLYCOPROTEIN GP120, T-cell surface glycoprotein CD4, ANTIBODY 17B, LIGHT CHAIN, ... (8 entities in total) |
| Functional Keywords | hiv, gp120, antibody, cd4, viral protein-immune system complex, viral protein/immune system |
| Biological source | Human immunodeficiency virus 1 More |
| Total number of polymer chains | 4 |
| Total formula weight | 106686.50 |
| Authors | Zhou, T.,Xu, L.,Dey, B.,Hessell, A.J.,Van Ryk, D.,Xiang, S.H.,Yang, X.,Zhang, M.Y.,Zwick, M.B.,Arthos, J.,Burton, D.R.,Dimitrov, D.S.,Sodroski, J.,Wyatt, R.,Nabel, G.J.,Kwong, P.D. (deposition date: 2006-11-20, release date: 2007-02-06, Last modification date: 2024-10-30) |
| Primary citation | Zhou, T.,Xu, L.,Dey, B.,Hessell, A.J.,Van Ryk, D.,Xiang, S.H.,Yang, X.,Zhang, M.Y.,Zwick, M.B.,Arthos, J.,Burton, D.R.,Dimitrov, D.S.,Sodroski, J.,Wyatt, R.,Nabel, G.J.,Kwong, P.D. Structural definition of a conserved neutralization epitope on HIV-1 gp120. Nature, 445:732-737, 2007 Cited by PubMed Abstract: The remarkable diversity, glycosylation and conformational flexibility of the human immunodeficiency virus type 1 (HIV-1) envelope (Env), including substantial rearrangement of the gp120 glycoprotein upon binding the CD4 receptor, allow it to evade antibody-mediated neutralization. Despite this complexity, the HIV-1 Env must retain conserved determinants that mediate CD4 binding. To evaluate how these determinants might provide opportunities for antibody recognition, we created variants of gp120 stabilized in the CD4-bound state, assessed binding of CD4 and of receptor-binding-site antibodies, and determined the structure at 2.3 A resolution of the broadly neutralizing antibody b12 in complex with gp120. b12 binds to a conformationally invariant surface that overlaps a distinct subset of the CD4-binding site. This surface is involved in the metastable attachment of CD4, before the gp120 rearrangement required for stable engagement. A site of vulnerability, related to a functional requirement for efficient association with CD4, can therefore be targeted by antibody to neutralize HIV-1. PubMed: 17301785DOI: 10.1038/nature05580 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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