2NY4
HIV-1 gp120 Envelope Glycoprotein (K231C, T257S, E268C, S334A, S375W) Complexed with CD4 and Antibody 17b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-13 |
Detector | MAR CCD 225 mm |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 70.963, 87.957, 196.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.910 - 2.000 |
R-factor | 0.20407 |
Rwork | 0.202 |
R-free | 0.24072 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gc1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.935 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.125 | 0.078 | 0.444 |
Number of reflections | 82010 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 97.9 | 99.7 | 87.5 |
Redundancy | 6.1 | 7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 7.0% PEG 8000, 8.0% MPD, 100 mM Na Citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |