2NVX

RNA polymerase II elongation complex in 5 mM Mg+2 with 2'-dUTP

2NVX の概要

• エントリーDOI: 10.2210/pdb2nvx/pdb
• 関連するPDBエントリー: 1R9S 1R9T 1SFO 2E2H 2E2I 2E2J 2NVQ 2NVS 2NVT 2NVY 2NVZ
• 分子名称: 5'-R(*AP*UP*CP*GP*AP*GP*AP*GP*GP*A)-3', DNA-directed RNA polymerase II subunit 9, DNA-directed RNA polymerases I/II/III subunit 10, ... (16 entities in total)
• 機能のキーワード: transcription, mrna, multiprotein complex, molecular machine, dna, transferase-dna-rna hybrid complex, transferase/dna-rna hybrid
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P08518 P16370 P20434 P20436 P27999 P38902; Nucleus, nucleolus: P40422 P22139; Cytoplasm: P20435
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 486727.04

構造登録者

Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D. (登録日: 2006-11-13, 公開日: 2006-12-19, 最終更新日: 2023-10-25)

主引用文献

Wang, D.,Bushnell, D.A.,Westover, K.D.,Kaplan, C.D.,Kornberg, R.D.
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Cell(Cambridge,Mass.), 127:941-954, 2006

PubMed Abstract: New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

PubMed: 17129781
DOI: 10.1016/j.cell.2006.11.023

実験手法

X-RAY DIFFRACTION (3.6 Å)