2NQ0

Solution Structures of a DNA Dodecamer Duplex with a Cisplatin 1,2-d(GG) Intrastrand Cross-Link

Summary for 2NQ0

• Entry DOI: 10.2210/pdb2nq0/pdb
• Related: 2NPW 2NQ1 2NQ4
• Descriptor: 5'-D(*CP*CP*TP*CP*AP*GP*GP*CP*CP*TP*CP*C)-3', 5'-D(*GP*GP*AP*GP*GP*CP*CP*TP*GP*AP*GP*G)-3', Cisplatin (3 entities in total)
• Functional Keywords: deoxyribonucleic acid, cisplatin, dna, duplex, dodecamer
• Total number of polymer chains: 2
• Total formula weight: 7627.82

Authors

Wu, Y.,Bhattacharyya, D.,Chaney, S.,Campbell, S. (deposition date: 2006-10-30, release date: 2007-06-12, Last modification date: 2023-12-27)

Primary citation

Wu, Y.,Bhattacharyya, D.,King, C.L.,Baskerville-Abraham, I.,Huh, S.-H.,Boysen, G.,Swenberg, J.A.,Temple, B.,Campbell, S.L.,Chaney, S.G.
Solution Structures of a DNA Dodecamer Duplex with and without a Cisplatin 1,2-d(GG) Intrastrand Cross-Link: Comparison with the Same DNA Duplex Containing an Oxaliplatin 1,2-d(GG) Intrastrand Cross-Link
Biochemistry, 46:6477-6487, 2007

PubMed Abstract: Proteins that discriminate between cisplatin-DNA adducts and oxaliplatin-DNA adducts are thought to be responsible for the differences in tumor range, toxicity, and mutagenicity of these two important chemotherapeutic agents. However, the structural basis for differential protein recognition of these adducts has not been determined and could be important for the design of more effective platinum anticancer agents. We have determined high-resolution NMR structures for cisplatin-GG and undamaged DNA dodecamers in the AGGC sequence context and have compared these structures with the oxaliplatin-GG structure in the same sequence context determined previously in our laboratory. This structural study allows the first direct comparison of cisplatin-GG DNA and oxaliplatin-GG DNA solution structures referenced to undamaged DNA in the same sequence context. Non-hydrogen atom rmsds of 0.81 and 1.21 were determined for the 15 lowest-energy structures for cisplatin-GG DNA and undamaged DNA, respectively, indicating good structural convergence. The theoretical NOESY spectra obtained by back-calculation from the final average structures showed excellent agreement with the experimental data, indicating that the final structures are consistent with the NMR data. Several significant conformational differences were observed between the cisplatin-GG adduct and the oxaliplatin-GG adduct, including buckle at the 5' G6.C19 base pair, opening at the 3' G7.C18 base pair, twist at the A5G6.T20C19 base pair step, slide, twist, and roll at the G6G7.C19C18 base pair step, slide at the G7C8.C18G17 base pair step, G6G7 dihedral angle, and overall bend angle. We hypothesize that these conformational differences may be related to the ability of various DNA repair proteins, DNA binding proteins, and DNA polymerases to discriminate between cisplatin-GG and oxaliplatin-GG adducts.

PubMed: 17497831
DOI: 10.1021/bi062291f

Experimental method

SOLUTION NMR