2NMV

Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA

Summary for 2NMV

• Entry DOI: 10.2210/pdb2nmv/pdb
• Related: 2D7D
• Descriptor: 5'-D(P*TP*TP*TP*TP*T)-3', UvrABC system protein B, N-METHYLACETAMIDE, ... (6 entities in total)
• Functional Keywords: protein-dna complex, t-fluorescein, hairpin, hydrolase-dna complex, hydrolase/dna
• Biological source: Bacillus subtilis; More
• Cellular location: Cytoplasm (By similarity): P37954 P37954
• Total number of polymer chains: 3
• Total formula weight: 83254.97

Authors

Waters, T.R.,Eryilmaz, J.,Geddes, S.,Barrett, T.E. (deposition date: 2006-10-23, release date: 2007-01-16, Last modification date: 2023-10-25)

Primary citation

Waters, T.R.,Eryilmaz, J.,Geddes, S.,Barrett, T.E.
Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA
Febs Lett., 580:6423-6427, 2006

PubMed Abstract: UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.

PubMed: 17097086
DOI: 10.1016/j.febslet.2006.10.051

Experimental method

X-RAY DIFFRACTION (2.95 Å)