2NMV

Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA

2NMV の概要

• エントリーDOI: 10.2210/pdb2nmv/pdb
• 関連するPDBエントリー: 2D7D
• 分子名称: 5'-D(P*TP*TP*TP*TP*T)-3', UvrABC system protein B, N-METHYLACETAMIDE, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, t-fluorescein, hairpin, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Bacillus subtilis; 詳細
• 細胞内の位置: Cytoplasm (By similarity): P37954 P37954
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 83254.97

構造登録者

Waters, T.R.,Eryilmaz, J.,Geddes, S.,Barrett, T.E. (登録日: 2006-10-23, 公開日: 2007-01-16, 最終更新日: 2023-10-25)

主引用文献

Waters, T.R.,Eryilmaz, J.,Geddes, S.,Barrett, T.E.
Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA
Febs Lett., 580:6423-6427, 2006

PubMed Abstract: UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.

PubMed: 17097086
DOI: 10.1016/j.febslet.2006.10.051

実験手法

X-RAY DIFFRACTION (2.95 Å)