2NLL

RETINOID X RECEPTOR-THYROID HORMONE RECEPTOR DNA-BINDING DOMAIN HETERODIMER BOUND TO THYROID RESPONSE ELEMENT DNA

Summary for 2NLL

• Entry DOI: 10.2210/pdb2nll/pdb
• Descriptor: DNA (5'-D(*CP*AP*GP*GP*TP*CP*AP*TP*TP*(5IU)P*CP*AP*GP*GP*TP*CP*AP*G)-3'), DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*GP*AP*AP*AP*TP*GP*AP*CP*CP*T P*G)-3'), PROTEIN (RETINOIC ACID RECEPTOR), ... (6 entities in total)
• Functional Keywords: complex (transcription regulation-dna), dna-binding, nuclear protein, zinc- finger, multigene family, alternative splicing, receptor, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P19793 P10828
• Total number of polymer chains: 4
• Total formula weight: 31357.99

Authors

Rastinejad, F.,Perlmann, T.,Evans, R.M.,Sigler, P.B. (deposition date: 1996-11-20, release date: 1997-03-12, Last modification date: 2024-02-21)

Primary citation

Rastinejad, F.,Perlmann, T.,Evans, R.M.,Sigler, P.B.
Structural determinants of nuclear receptor assembly on DNA direct repeats.
Nature, 375:203-211, 1995

PubMed Abstract: Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5'-AGGTCA-3' separated by one to five base pairs. The 1.9 A crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.

PubMed: 7746322
DOI: 10.1038/375203a0

Experimental method

X-RAY DIFFRACTION (1.9 Å)