2NL9
Crystal structure of the Mcl-1:Bim BH3 complex
Summary for 2NL9
| Entry DOI | 10.2210/pdb2nl9/pdb |
| Related | 1WSX 2NLA |
| Descriptor | FUSION PROTEIN CONSISTING OF Induced myeloid leukemia cell differentiation protein Mcl-1 homolog, Bcl-2-like protein 11, ZINC ION, ... (5 entities in total) |
| Functional Keywords | apoptosis, bcl-2, mcl-1, bim |
| Biological source | Mus musculus (Mouse) More |
| Cellular location | Membrane ; Single-pass membrane protein : Q07820 Endomembrane system ; Peripheral membrane protein . Isoform BimEL: Mitochondrion. Isoform BimL: Mitochondrion. Isoform BimS: Mitochondrion. Isoform Bim-alpha1: Mitochondrion: O43521 |
| Total number of polymer chains | 2 |
| Total formula weight | 21677.93 |
| Authors | Czabotar, P.E.,Colman, P.M. (deposition date: 2006-10-19, release date: 2007-03-27, Last modification date: 2024-10-30) |
| Primary citation | Czabotar, P.E.,Lee, E.F.,van Delft, M.F.,Day, C.L.,Smith, B.J.,Huang, D.C.,Fairlie, W.D.,Hinds, M.G.,Colman, P.M. Structural insights into the degradation of Mcl-1 induced by BH3 domains. Proc.Natl.Acad.Sci.USA, 104:6217-6222, 2007 Cited by PubMed Abstract: Apoptosis is held in check by prosurvival proteins of the Bcl-2 family. The distantly related BH3-only proteins bind to and antagonize them, thereby promoting apoptosis. Whereas binding of the BH3-only protein Noxa to prosurvival Mcl-1 induces Mcl-1 degradation by the proteasome, binding of another BH3-only ligand, Bim, elevates Mcl-1 protein levels. We compared the three-dimensional structures of the complexes formed between BH3 peptides of both Bim and Noxa, and we show that a discrete C-terminal sequence of the Noxa BH3 is necessary to instigate Mcl-1 degradation. PubMed: 17389404DOI: 10.1073/pnas.0701297104 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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