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2NAX

Structure of CCHC zinc finger domain of Pcf11

Summary for 2NAX
Entry DOI10.2210/pdb2nax/pdb
NMR InformationBMRB: 25953
DescriptorProtein PCF11, ZINC ION (2 entities in total)
Functional Keywordszinc finger, metal binding protein
Biological sourceSaccharomyces cerevisiae S288c (Baker's yeast)
Cellular locationNucleus : P39081
Total number of polymer chains1
Total formula weight8720.82
Authors
Yang, F.,Varani, G. (deposition date: 2016-01-12, release date: 2016-11-23, Last modification date: 2024-05-15)
Primary citationYang, F.,Hsu, P.,Lee, S.D.,Yang, W.,Hoskinson, D.,Xu, W.,Moore, C.,Varani, G.
The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing.
RNA, 23:98-107, 2017
Cited by
PubMed Abstract: 3'-End processing of pre-mRNAs prior to packaging and export to the cytoplasm of the mature transcript is a highly regulated process executed by several tens of protein factors that recognize poorly conserved RNA signals. Among them is Pcf11, a highly conserved, multidomain protein that links transcriptional elongation, 3'-end processing, and transcription termination. Here we report the structure and biochemical function of Pcf11's C-terminal domain, which is conserved from yeast to humans. We identify a novel zinc-finger fold, resembling a trillium flower. Structural, biochemical, and genetic analyses reveal a highly conserved surface that plays a critical role in both cleavage and polyadenylation. These findings provide further insight into this important protein and its multiple functional roles during cotranscriptional RNA processing.
PubMed: 27780845
DOI: 10.1261/rna.058354.116
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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