2N8V
An NMR/SAXS structure of the PKI domain of the honeybee dicistrovirus, Israeli acute paralysis virus (IAPV) IRES
Summary for 2N8V
| Entry DOI | 10.2210/pdb2n8v/pdb |
| NMR Information | BMRB: 25867 |
| Descriptor | RNA (70-MER) (1 entity in total) |
| Functional Keywords | rna, iapv, pseudoknot |
| Biological source | Apis mellifera |
| Total number of polymer chains | 1 |
| Total formula weight | 22565.44 |
| Authors | Au, H.H.,Cornilescu, G.,Mouzakis, K.D.,Burke, J.E.,Ren, Q.,Lee, S.,Butcher, S.E.,Jan, E. (deposition date: 2015-10-27, release date: 2015-11-11, Last modification date: 2024-05-01) |
| Primary citation | Au, H.H.,Cornilescu, G.,Mouzakis, K.D.,Ren, Q.,Burke, J.E.,Lee, S.,Butcher, S.E.,Jan, E. Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection. Proc.Natl.Acad.Sci.USA, 112:E6446-E6455, 2015 Cited by PubMed Abstract: The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts a triple-pseudoknotted RNA structure and occupies the core ribosomal E, P, and A sites to directly recruit the ribosome and initiate translation at a non-AUG codon. A subset of dicistrovirus IRESs directs translation in the 0 and +1 frames to produce the viral structural proteins and a +1 overlapping open reading frame called ORFx, respectively. Here we show that specific mutations of two unpaired adenosines located at the core of the three-helical junction of the honey bee dicistrovirus Israeli acute paralysis virus (IAPV) IRES PKI domain can uncouple 0 and +1 frame translation, suggesting that the structure adopts distinct conformations that contribute to 0 or +1 frame translation. Using a reconstituted translation system, we show that ribosomes assembled on mutant IRESs that direct exclusive 0 or +1 frame translation lack reading frame fidelity. Finally, a nuclear magnetic resonance/small-angle X-ray scattering hybrid approach reveals that the PKI domain of the IAPV IRES adopts an RNA structure that resembles a complete tRNA. The tRNA shape-mimicry enables the viral IRES to gain access to the ribosome tRNA-binding sites and form intermolecular contacts with the ribosome that are necessary for initiating IRES translation in a specific reading frame. PubMed: 26554019DOI: 10.1073/pnas.1512088112 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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