2N4Y

Structure and possible function of a G-quadruplex in the long terminal repeat of the proviral HIV-1 genome

Summary for 2N4Y

• Entry DOI: 10.2210/pdb2n4y/pdb
• NMR Information: BMRB: 25686
• Descriptor: DNA_(5'-D(*CP*TP*GP*GP*GP*CP*GP*GP*GP*AP*CP*TP*GP*GP*GP*GP*AP*GP*TP*GP*GP*T)-3') (1 entity in total)
• Functional Keywords: g-quadruplex, hiv-1, long terminal repeat, dna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 6945.45

Authors

DeNicola, B.,Lech, C.J.,Heddi, B.,Regmi, S.,Frasson, I.,Perrone, R.,Richter, S.N.,Phan, A.T. (deposition date: 2015-07-02, release date: 2016-06-29, Last modification date: 2024-05-15)

Primary citation

De Nicola, B.,Lech, C.J.,Heddi, B.,Regmi, S.,Frasson, I.,Perrone, R.,Richter, S.N.,Phan, A.T.
Structure and possible function of a G-quadruplex in the long terminal repeat of the proviral HIV-1 genome.
Nucleic Acids Res., 44:6442-6451, 2016

PubMed Abstract: The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection. The guanine-rich U3 region within the LTR promoter, previously shown to form G-quadruplex structures, represents an attractive target to inhibit HIV transcription and replication. In this work, we report the structure of a biologically relevant G-quadruplex within the LTR promoter region of HIV-1. The guanine-rich sequence designated LTR-IV forms a well-defined structure in physiological cationic solution. The nuclear magnetic resonance (NMR) structure of this sequence reveals a parallel-stranded G-quadruplex containing a single-nucleotide thymine bulge, which participates in a conserved stacking interaction with a neighboring single-nucleotide adenine loop. Transcription analysis in a HIV-1 replication competent cell indicates that the LTR-IV region may act as a modulator of G-quadruplex formation in the LTR promoter. Consequently, the LTR-IV G-quadruplex structure presented within this work could represent a valuable target for the design of HIV therapeutics.

PubMed: 27298260
DOI: 10.1093/nar/gkw432

Experimental method

SOLUTION NMR