2MZV

Resonance assignments and secondary structure of a phytocystatin from Sesamum indicum

Summary for 2MZV

• Entry DOI: 10.2210/pdb2mzv/pdb
• NMR Information: BMRB: 19689
• Descriptor: Cystatin (1 entity in total)
• Functional Keywords: hydrolase inhibitor
• Biological source: Sesamum indicum (beniseed,gingelly,hu ma,koba)
• Total number of polymer chains: 1
• Total formula weight: 22389.27

Authors

Chyan, C. (deposition date: 2015-02-25, release date: 2016-03-02, Last modification date: 2024-05-15)

Primary citation

Hu, Y.J.,Irene, D.,Lo, C.J.,Cai, Y.L.,Tzen, T.C.,Lin, T.H.,Chyan, C.L.
Resonance assignments and secondary structure of a phytocystatin from Sesamum indicum.
Biomol.Nmr Assign., 9:309-311, 2015

PubMed Abstract: A cDNA encoding a cysteine protease inhibitor, cystatin was cloned from sesame (Sesamum indicum L.) seed. This clone was constructed into an expression vector and expressed in E. coli and purified to homogeneous. The recombinant sesame cystatin (SiCYS) showed effectively inhibitory activity toward C1 cysteine proteases. In order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear NMR techniques were used to characterize the structure of SiCYS. The full (1)H, (15)N, and (13)C resonances of SiCYS were assigned. The secondary structure of SiCYS was identified by using the assigned chemical shifts of (1)H(α), (13)C(α), (13)C(β), and (13)CO through the consensus chemical shift index (CSI). The results of CSI analysis of SiCYS suggest eight β-strands (residues 33-46, 51-61, 63-75, 80-87, 150-155, 157-169, 172-183, and 192-195) and two α-helices (residues 16-30, and 120-135).

PubMed: 25673506
DOI: 10.1007/s12104-015-9598-y

Experimental method

SOLUTION NMR