2MZN

NMR structure of the HLTF HIRAN domain in its DNA-bound conformation

Summary for 2MZN

• Entry DOI: 10.2210/pdb2mzn/pdb
• NMR Information: BMRB: 25492
• Descriptor: Helicase-like transcription factor (1 entity in total)
• Functional Keywords: hiran, hltf, dna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : Q14527
• Total number of polymer chains: 1
• Total formula weight: 13532.33

Authors

Korzhnev, D.,Eldirany, S. (deposition date: 2015-02-18, release date: 2015-07-01, Last modification date: 2024-05-15)

Primary citation

Kile, A.C.,Chavez, D.A.,Bacal, J.,Eldirany, S.,Korzhnev, D.M.,Bezsonova, I.,Eichman, B.F.,Cimprich, K.A.
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Mol.Cell, 58:1090-1100, 2015

PubMed Abstract: Stalled replication forks are a critical problem for the cell because they can lead to complex genome rearrangements that underlie cell death and disease. Processes such as DNA damage tolerance and replication fork reversal protect stalled forks from these events. A central mediator of these DNA damage responses in humans is the Rad5-related DNA translocase, HLTF. Here, we present biochemical and structural evidence that the HIRAN domain, an ancient and conserved domain found in HLTF and other DNA processing proteins, is a modified oligonucleotide/oligosaccharide (OB) fold that binds to 3' ssDNA ends. We demonstrate that the HIRAN domain promotes HLTF-dependent fork reversal in vitro through its interaction with 3' ssDNA ends found at forks. Finally, we show that HLTF restrains replication fork progression in cells in a HIRAN-dependent manner. These findings establish a mechanism of HLTF-mediated fork reversal and provide insight into the requirement for distinct fork remodeling activities in the cell.

PubMed: 26051180
DOI: 10.1016/j.molcel.2015.05.013

Experimental method

SOLUTION NMR