2MZD

Characterization of the p300 Taz2-p53 TAD2 Complex and Comparison with the p300 Taz2-p53 TAD1 Complex

2MZD の概要

• エントリーDOI: 10.2210/pdb2mzd/pdb
• 関連するPDBエントリー: 2K8F
• NMR情報: BMRB: 25484
• 分子名称: Histone acetyltransferase p300, Cellular tumor antigen p53 (2 entities in total)
• 機能のキーワード: protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q09472; Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12814.86

構造登録者

Miller Jenkins, L.M.,Feng, H.,Durell, S.R.,Tagad, H.D.,Mazur, S.J.,Tropea, J.E.,Bai, Y.,Appella, E. (登録日: 2015-02-11, 公開日: 2015-03-25, 最終更新日: 2024-05-15)

主引用文献

Miller Jenkins, L.M.,Feng, H.,Durell, S.R.,Tagad, H.D.,Mazur, S.J.,Tropea, J.E.,Bai, Y.,Appella, E.
Characterization of the p300 Taz2-p53 TAD2 Complex and Comparison with the p300 Taz2-p53 TAD1 Complex.
Biochemistry, 54:2001-2010, 2015

PubMed Abstract: The p53 tumor suppressor is a critical mediator of the cellular response to stress. The N-terminal transactivation domain of p53 makes protein interactions that promote its function as a transcription factor. Among those cofactors is the histone acetyltransferase p300, which both stabilizes p53 and promotes local chromatin unwinding. Here, we report the nuclear magnetic resonance solution structure of the Taz2 domain of p300 bound to the second transactivation subdomain of p53. In the complex, p53 forms an α-helix between residues 47 and 55 that interacts with the α1-α2-α3 face of Taz2. Mutational analysis indicated several residues in both p53 and Taz2 that are critical for stabilizing the interaction. Finally, further characterization of the complex by isothermal titration calorimetry revealed that complex formation is pH-dependent and releases a bound chloride ion. This study highlights differences in the structures of complexes formed by the two transactivation subdomains of p53 that may be broadly observed and play critical roles in p53 transcriptional activity.

PubMed: 25753752
DOI: 10.1021/acs.biochem.5b00044

実験手法

SOLUTION NMR